Natalee Holloway's mother is seeking at least $35 million from the producers of what she says was a fake television documentary about the case, according to TMZ.
Natalee was 18- years-old when she was last seen during a trip with classmates in Aruba in 2005.
Her mysterious disappearance after a night with friends at a nightclub sparked years of news coverage, particularly in the tabloid and true-crime media.
Beth Holloway said in a federal lawsuit that the deception surrounding "The Disappearance of Natalee Holloway" documentary was so convincing, that she was tricked into providing a DNA sample; to match against what producers claimed were remains that could be those of her long-missing daughter.
According to the suit, The whole show was a ruse that subjected Beth Holloway to "agonizing weeks" of uncertainty and waiting that "completely and utterly destroyed" her.
Holloway, a schoolteacher in north Alabama, is seeking $10 million in compensation and $25 million in punitive damages against Oxygen Media, an arm of NBCUniversal Cable Entertainment, and the Los Angeles-based Brian Graden Media.
The six-episode series aired last year by Oxygen included the discovery of what were supposedly remains that could be those of Natalee.
But the lawsuit claims that producers knew that bone fragments featured in the production weren't linked to Natalee before supposed testing produced inconclusive results.
Rather than being a documentary or true investigation, the show was a "scripted, pre-planned farce calculated to give the impression of real-time events," the suit contends.
Natalee Holloway's father Dave Holloway participated in the program and contacted Beth Holloway seeking a DNA sample for use in testing, the complaint said. Dave Holloway isn't listed among the defendants.
Holloway's remains were never found and Joran van der Sloot, the Dutch teen suspected in her death, is now imprisoned for the slaying of another woman killed in 2010.
A judge acting at Dave Holloway's request declared Natalee Holloway legally dead six years ago.